Tender №C02572 for microbiological tests using the molecular method of counting bacteria and archaea (qPCR).

- Microbiological tests using the molecular method of counting bacteria and archaea (qPCR).

1.      Availability of laboratory, qualified personnel, equipment, materials for tests as well as collection/library/database of bacteria types typical for the oil and gas industry.

2.      The studies to be carried out shall include:

·        Microbiological methods of separation and cultivation of aerobic and anaerobic bacteria and archaea.

·        Conducting microbiological tests using the molecular method of counting bacteria and archaea – quantitative PCR* (qPCR), including 16s DNA Metagenomic test.

·        Methods for studying morphology, analysis of 16s DNA genes, etc., necessary for identifying and describing prokaryotes. Using the same set of primers for qPCR as for 16s DNA Metagenomic.

·        Assessment of the rate of sulphate reduction, nitrate reduction, methane producing and oxidation in produced and process water samples.

·        Microbiological studies of formation water, sediments, and deposits, determination of the structure and composition of microbial biofilms.

·        Assessment of biodiversity and efficiency of microorganism control at oil and water production, treatment and transportation facilities.

·        Determination of the activity and efficiency of chemical compounds (biocides) against bacteria.